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Human PCX (Podocalyxin) ELISA Kit – E-EL-H2360

96 rx

797.00$ CAD

0 in stock (expect 7-14 days for delivery)

0 in stock (expect 7-14 days for delivery)

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SKU: E-EL-H2360 Category: Tags: , Brand:

Human PCX (Podocalyxin) ELISA Kit

The Human PCX ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PCX. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for PCX and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PCX, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of PCX. You can calculate the concentration of PCX in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Podocalyxin

Abbreviation : PCX

Uniprot ID : O00592

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

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96 rx

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