Human PAFAHIB3 (Platelet Activating Factor Acetylhydrolase Ib3) ELISA Kit – E-EL-H2200

Human PAFAHIB3 (Platelet Activating Factor Acetylhydrolase Ib3) ELISA Kit

The Human PAFAHIB3 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PAFAHIB3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PAFAHIB3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAFAHIB3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PAFAHIB3. You can calculate the concentration of Human PAFAHIB3 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Platelet Activating Factor Acetylhydrolase Ib3

Abbreviation : PAFAHIB3

Uniprot ID :

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

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