Human OSM (Oncostatin M) ELISA Kit – E-EL-H2247

Human OSM (Oncostatin M) ELISA Kit

The Human OSM ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to OSM. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for OSM and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain OSM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of OSM. You can calculate the concentration of OSM in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Oncostatin M

Abbreviation : OSM

Uniprot ID : P13725

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

More information about this Human OSM (Oncostatin M) ELISA Kit from Elabscience