Human Ntn4 (Netrin 4) ELISA Kit – E-EL-H2329

Human Ntn4 (Netrin 4) ELISA Kit

The Human Ntn4 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Ntn4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Ntn4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Ntn4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Ntn4. You can calculate the concentration of Ntn4 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Netrin 4

Abbreviation : Ntn4

Uniprot ID : Q9HB63

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 46.875 pg/ml

Detection Range : 78.125 – 5000 pg/ml

Manual & Datasheet

More information about this Human Ntn4 (Netrin 4) ELISA Kit from Elabscience