Human NADPH (Nicotinamide Adenine Dinucleotide Phosphate) ELISA Kit – E-EL-H2221

Human NADPH (Nicotinamide Adenine Dinucleotide Phosphate) ELISA Kit

The Human NADPH ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with Human NADPH. During the reaction, Human NADPH in the sample or standard competes with a fixed amount of Human NADPH on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to Human NADPH. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human NADPH in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Nicotinamide Adenine Dinucleotide Phosphate

Abbreviation : NADPH

Uniprot ID :

Format : 96 rx

Reactivity : Human

ELISA configuration : Competitive

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

More information about this Human NADPH (Nicotinamide Adenine Dinucleotide Phosphate) ELISA Kit from Elabscience