Human MHCC/HLA-C (Major Histocompatibility Complex Class I C) ELISA Kit – E-EL-H2331

Human MHCC/HLA-C (Major Histocompatibility Complex Class I C) ELISA Kit

The Human MHCC/HLA-C ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MHCC/HLA-C. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for MHCC/HLA-C and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MHCC/HLA-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of MHCC/HLA-C. You can calculate the concentration of MHCC/HLA-C in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Major Histocompatibility Complex Class I C

Abbreviation : MHCC/HLA-C

Uniprot ID : P10321

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

More information about this Human MHCC/HLA-C (Major Histocompatibility Complex Class I C) ELISA Kit from Elabscience