Human IDO1 / IDO Protein (His tag) – PKSH030356

Recombinant Human IDO1 / IDO Protein (His tag)

Indoleamine 2,3-dioxygenase-1, also known as Indoleamine-pyrrole 2,3-dioxygenase, IDO1 and IDO, is a member of the indoleamine 2,3-dioxygenase family. IDO1 / IDO and tryptophan 2,3-dioxygenase (TDO) are tryptophan-degrading enzymes that catalyze the first step in tryptophan catabolism via the kynurenine pathway. TDO is widely distributed in both eukaryotes and bacteria. In contrast, IDO has been found only in mammals and yeast. In 2007, a third enzyme, indoleamine 2,3-dioxygenase-2 (IDO2), was discovered. IDO2 is found not only in mammals but also in lower vertebrates. IDO1 / IDO is an immunosuppressive molecule inducible in various cells. IDO1 / IDO catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. It mediates oxidative cleavage of tryptophan, an amino acid essential for cell proliferation and survival. IDO1 / IDO inhibition is proposed to have therapeutic potential in immunodeficiency-associated abnormalities, including cancer. The IDO pathway is activated in multiple tumor types. Selective inhibition of IDO1 may represent an attractive cancer therapeutic strategy via up-regulation of cellular immunity. IDO1 / IDO is an enzyme that suppresses adaptive T-cell immunity by catabolizing tryptophan from the cellular microenvironment. Inhibition of IDO pathway might enhance the efficacy of immunotherapeutic strategies for cancer.

Synonym(s) : IDO , INDO

Species(s) : Human

Protein Expression Host : E. coli

Protein Construction : A DNA sequence encoding the human IDO1 (P14902-1) (Ala 2-Gly 403) was expressed, with a polyhistide tag at the N-terminus.

Predicted N-terminal amino acid : Met

Molecular weight/mass : The recombinant human IDO1 consisting of 409 amino acids and has a calculated molecular mass of 46 kDa. It migrates as an approximately 44 kDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the human IDO1 (P14902-1) (Ala 2-Gly 403) was expressed, with a polyhistide tag at the N-terminus.

Formulation : Supplied as sterile 50mM Tris, 0.15M NaCl, 10% glycerolcerol, pH 8.0

Purity : > 88 % as determined by SDS-PAGE

Endotoxin level :

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : Liquid. It is shipped out with blue ice.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet