Human HbC (Hemoglobin C) ELISA Kit – E-EL-H2169

Human HbC (Hemoglobin C) ELISA Kit

The Human HbC ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HbC. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for HbC and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HbC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of HbC. You can calculate the concentration of HbC in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Hemoglobin C

Abbreviation : HbC

Uniprot ID :

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.938 ng/ml

Detection Range : 1.563 – 100 ng/ml

Manual & Datasheet

More information about this Human HbC (Hemoglobin C) ELISA Kit from Elabscience