Human GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) ELISA kit
The Human GAPDH ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GAPDH. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human GAPDH and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAPDH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GAPDH. You can calculate the concentration of Human GAPDH in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Glyceraldehyde-3-Phosphate Dehydrogenase
Abbreviation : GAPDH
Uniprot ID : P04406
Format : 96 rx
Reactivity : Human
ELISA configuration : Sandwich
Sensitivity : 0.938 ng/ml
Detection Range : 1.563 – 100 ng/ml
Additional information
Format | 96 rx |
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