Human GAD65 / GAD2 / GAD-2 Protein (GST Tag) – PKSH030280

Recombinant Human GAD65 / GAD2 / GAD-2 Protein (GST Tag)

Mouse glutamate decarboxylase 2, also known as glutamate decarboxylase 65 kDa isoform, 65 kDa glutamic acid decarboxylase, GAD2 and GAD65, is a member of the group II decarboxylase family. GAD2 is identified as a major autoantigen in insulin-dependent diabetes. GAD2 is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. GAD2 may also play a role in the stiff man syndrome. GAD2 is implicated in the formation of the gamma-aminobutyric acid (GABA), a neurotransmitter involved in the regulation of food intake. GABA is synthesized in brain by two isoforms of glutamic acid decarboxylase (Gad), GAD1 and GAD2. GAD1 provides most of the GABA in brain, but GAD2 can be rapidly activated in times of high GABA demand. Mice lacking GAD2 are viable whereas deletion of GAD1 is lethal. Deletion of GAD2 increased ethanol palatability and intake and slightly reduced the severity of ethanol-induced withdrawal.

Synonym(s) : GAD65

Species(s) : Human

Protein Expression Host : Baculovirus-Insect Cells

Protein Construction : A DNA sequence encoding the human GAD2 (NP_000809.1) (Met1-Leu585) was expressed with a GST tag at the N-terminus.

Predicted N-terminal amino acid : Met

Molecular weight/mass : The recombinant human GAD2 consists of 819 amino acids and predicts a molecular mass of 92.6 kDa.

Protein Construction : A DNA sequence encoding the human GAD2 (NP_000809.1) (Met1-Leu585) was expressed with a GST tag at the N-terminus.

Formulation : Lyophilized from sterile 200 Tirs, 150 mM NaCl, pH 8.0, 10 % glycerol, 1 mM GSH

Purity : > 90 % as determined by SDS-PAGE.

Endotoxin level : <1.0 EU per μg protein as determined by the LAL method.

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet