Human FG (Fibrinogen) ELISA Kit – E-EL-H2193

Human FG (Fibrinogen) ELISA Kit

The Human FG ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FG. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for FG and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of FG. You can calculate the concentration of FG in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Fibrinogen

Abbreviation : FG

Uniprot ID : 

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 5.625 ng/ml

Detection Range : 9.375 – 600 ng/ml

Manual & Datasheet

More information about this Human FG (Fibrinogen) ELISA Kit from Elabscience