Human eIF3a (Eukaryotic Translation Initiation Factor 3a) ELISA Kit – E-EL-H2283

Human eIF3a (Eukaryotic Translation Initiation Factor 3a) ELISA Kit

The Human eIF3a ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human eIF3a. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human eIF3a and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human eIF3a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human eIF3a. You can calculate the concentration of Human eIF3a in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Eukaryotic Translation Initiation Factor 3a

Abbreviation : eIF3a

Uniprot ID : Q14152

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 37.5 pg/ml

Detection Range : 62.5 – 4000 pg/ml

Manual & Datasheet

More information about this Human eIF3a (Eukaryotic Translation Initiation Factor 3a) ELISA Kit from Elabscience