Human DKK1 / Dkk-1 Protein (His tag) – PKSH031807

Recombinant Human DKK1 / Dkk-1 Protein (His tag)

Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.

Synonym(s) : SK , DKK-1

Species(s) : Human

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the human DKK1 precursor (NP_036374.1) (Met 2-His 266) was expressed with a C-terminal polyhistidine tag.

Predicted N-terminal amino acid : Thr 32 or Ser 35

Molecular weight/mass : The recombinant human DKK1 protein consists of 235 amino acids and has a calculated molecular mass of 25.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh DKK1 is approximately 45 kDa due to glycosylation.

Protein Construction : A DNA sequence encoding the human DKK1 precursor (NP_036374.1) (Met 2-His 266) was expressed with a C-terminal polyhistidine tag.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 90 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet