Human CDK5 (Cyclin Dependent Kinase 5) ELISA Kit – E-EL-H2324

Human CDK5 (Cyclin Dependent Kinase 5) ELISA Kit

The Human CDK5 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CDK5. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for CDK5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CDK5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CDK5. You can calculate the concentration of CDK5 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Cyclin Dependent Kinase 5

Abbreviation : CDK5

Uniprot ID : Q00535

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

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