Recombinant Human AMPK (G1/B2/A1) Heterotrimer Protein
Synonym(s) : AMPK , 5′ AMP-activated protein kinase , 5′ adenosine monophosphate-activated protein kinase. Heterotrimer of alpha 1 catalytic subunit , beta 2 non-catalytic subunit and gamma 1 non-catalytic subunit.
Species(s) : Human
Protein Expression Host : Baculovirus-Insect Cells
Protein Construction : A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
Predicted N-terminal amino acid : Met & Met & His
Molecular weight/mass : The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB2 / PRKAA1) has a calculated molecular mass of 160 (38+30+92) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions.
Protein Construction : A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
Formulation : Supplied as sterile 50mM Tris, 200mM NaCl, 2mM GSH, 1mM EDTA, 1mM DTT, 10% glycerol, pH 7.4
Purity : > 94 % as determined by SDS-PAGE
Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method
Stability : Samples are stable for up to twelve months from date of receipt at -70°C
Shipping conditions : Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Reconstitution : Please refer to the printed manual for detailed information.
Additional information
Format | 20 ug, 50 ug |
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Species | Human |
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