Human AIM2 (Absent In Melanoma 2) ELISA Kit – E-EL-H2427

Human AIM2 (Absent In Melanoma 2) ELISA Kit

The Human AIM2 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AIM2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for AIM2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AIM2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of AIM2. You can calculate the concentration of AIM2 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Absent In Melanoma 2

Abbreviation : AIM2

Uniprot ID : O14862

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

More information about this Human AIM2 (Absent In Melanoma 2) ELISA Kit from Elabscience