Human AIF1 (Allograft Inflammatory Factor 1) ELISA Kit – E-EL-H0302

Human AIF1 (Allograft Inflammatory Factor 1) ELISA Kit

The Human AIF1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AIF1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for AIF1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AIF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of AIF1. You can calculate the concentration of AIF1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Allograft Inflammatory Factor 1

Abbreviation : AIF1

Uniprot ID : P55008

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

More information about this Human AIF1 (Allograft Inflammatory Factor 1) ELISA Kit from Elabscience