Human AGR2 (Anterior Gradient Protein 2) ELISA Kit – E-EL-H0298

Human AGR2 (Anterior Gradient Protein 2) ELISA Kit

The Human AGR2 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AGR2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for AGR2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AGR2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of AGR2. You can calculate the concentration of AGR2 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Anterior Gradient Protein 2

Abbreviation : AGR2

Uniprot ID : O95994

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

More information about this Human AGR2 (Anterior Gradient Protein 2) ELISA Kit from Elabscience