Human AGGF1 (Angiogenic Factor with G Patch and FHA Domains 1) ELISA Kit – E-EL-H0188

Human AGGF1 (Angiogenic Factor with G Patch and FHA Domains 1) ELISA Kit

The Human AGGF1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AGGF1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for AGGF1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AGGF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of AGGF1. You can calculate the concentration of AGGF1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Angiogenic Factor with G Patch and FHA Domains 1

Abbreviation : AGGF1

Uniprot ID : Q8N302

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

More information about this Human AGGF1 (Angiogenic Factor with G Patch and FHA Domains 1) ELISA Kit from Elabscience