Human AGER (Advanced Glycosylation End Product Specific Receptor) ELISA Kit – E-EL-H0295

Human AGER (Advanced Glycosylation End Product Specific Receptor) ELISA Kit

The Human AGER ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AGER/RAGE. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for AGER/RAGE and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AGER/RAGE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of AGER/RAGE. You can calculate the concentration of AGER/RAGE in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Advanced Glycosylation End Product Specific Receptor

Abbreviation : AGER

Uniprot ID : Q15109

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 46.875 pg/ml

Detection Range : 78.125 – 5000 pg/ml

Manual & Datasheet

More information about this Human AGER (Advanced Glycosylation End Product Specific Receptor) ELISA Kit from Elabscience