Human AGC (Aggrecan) ELISA Kit – E-EL-H0294

Human AGC (Aggrecan) ELISA Kit

The Human AGC ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AGC. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for AGC and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AGC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of AGC. You can calculate the concentration of AGC in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Aggrecan

Abbreviation : AGC

Uniprot ID : P16112

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

More information about this Human AGC (Aggrecan) ELISA Kit from Elabscience