Human AFAP1 (Actin Filament Associated Protein 1) ELISA Kit – E-EL-H0286

Human AFAP1 (Actin Filament Associated Protein 1) ELISA Kit

The Human AFAP1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AFAP1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human AFAP1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AFAP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AFAP1. You can calculate the concentration of Human AFAP1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Actin Filament Associated Protein 1

Abbreviation : AFAP1

Uniprot ID : Q8N556

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.469 ng/ml

Detection Range : 0.781 – 50 ng/ml

Manual & Datasheet

More information about this Human AFAP1 (Actin Filament Associated Protein 1) ELISA Kit from Elabscience