Human AFA (Anti-Filaggrin Antibody) ELISA Kit – E-EL-H0283

Human AFA (Anti-Filaggrin Antibody) ELISA Kit

The Human AFA ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to Human AFA. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antigen. Then a biotinylated detection antigen specific for Human AFA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AFA, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AFA. You can calculate the concentration of Human AFA in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Anti-Filaggrin

Abbreviation : FLG-Ab

Uniprot ID : 

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.938 ng/ml

Detection Range : 1.563 – 100 ng/ml

Manual & Datasheet

More information about this Human AFA (Anti-Filaggrin Antibody) ELISA Kit from Elabscience