Human ADAM9 (A Disintegrin And Metalloprotease 9) ELISA Kit
The Human ADAM9 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ADAM9. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ADAM9 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ADAM9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ADAM9. You can calculate the concentration of ADAM9 in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: A Disintegrin And Metalloprotease 9
Abbreviation : ADAM9
Uniprot ID : Q13443
Format : 96 rx
Reactivity : Human
ELISA configuration : Sandwich
Sensitivity : 37.5 pg/ml
Detection Range : 62.5 – 4000 pg/ml
Additional information
Format | 96 rx |
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