Human ADAM10 (A Disintegrin And Metalloprotease 10) ELISA Kit – E-EL-H0263

Human ADAM10 (A Disintegrin And Metalloprotease 10) ELISA Kit

The Human ADAM10 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ADAM10. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ADAM10 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ADAM10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ADAM10. You can calculate the concentration of ADAM10 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: A Disintegrin And Metalloprotease 10

Abbreviation : ADAM10

Uniprot ID : O14672

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 37.5 pg/ml

Detection Range : 62.5 – 4000 pg/ml

Manual & Datasheet

More information about this Human ADAM10 (A Disintegrin And Metalloprotease 10) ELISA Kit from Elabscience