Human ACTN4 (Actinin Alpha 4) ELISA Kit
The Human ACTN4 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ACTN4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ACTN4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ACTN4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ACTN4. You can calculate the concentration of ACTN4 in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Actinin Alpha 4
Abbreviation : ACTN4
Uniprot ID : O43707
Format : 96 rx
Reactivity : Human
ELISA configuration : Sandwich
Sensitivity : 0.469 ng/ml
Detection Range : 0.781 – 50 ng/ml
More information about this Human ACTN4 (Actinin Alpha 4) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
---|
Ask a question about Human ACTN4 (Actinin Alpha 4) ELISA Kit – E-EL-H1636
You must be logged in to post a review.
Reviews