Human ACTH (Adrenocorticotropic Hormone) ELISA Kit – E-EL-H0137

Human ACTH (Adrenocorticotropic Hormone) ELISA Kit

The Human ACTH ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with ACTH. During the reaction, Human ACTH in the sample or standard competes with a fixed amount of ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human ACTH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human ACTH in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Adrenocorticotropic Hormone

Abbreviation : ACTH

Uniprot ID :

Format : 96 rx

Reactivity : Human

ELISA configuration : Competitive

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

More information about this Human ACTH (Adrenocorticotropic Hormone) ELISA Kit from Elabscience