Human ACP5 (Tartrate Resistant Acid Phosphatase 5) ELISA Kit – E-EL-H1551

Human ACP5 (Tartrate Resistant Acid Phosphatase 5) ELISA Kit

The Human ACP5 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ACP5. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ACP5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ACP5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ACP5. You can calculate the concentration of ACP5 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Tartrate Resistant Acid Phosphatase 5

Abbreviation : ACP5

Uniprot ID : P13686

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.469 ng/ml

Detection Range : 0.781 – 50 ng/ml

Manual & Datasheet

More information about this Human ACP5 (Tartrate Resistant Acid Phosphatase 5) ELISA Kit from Elabscience