Human ACK1 / TNK2 Protein (GST tag) – PKSH030470

Recombinant Human ACK1 / TNK2 Protein (GST tag)

ACK1 (also known as ACK, TNK2, or activated Cdc42 kinase) is a structurally unique non-receptor tyrosine kinase that is expressed in diverse cell types. This downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. The ACK1 protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. ACK1 integrates signals from plethora of ligand-activated receptor tyrosine kinases (RTKs), for example, MERTK, EGFR, HER2 and PDGFR to initiate intracellular signaling cascades. It binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. ACK1 transduces extracellular signals to cytosolic and nuclear effectors such as the protein kinase AKT/PKB and androgen receptor (AR), to promote cell survival and growth. ACK1 participates in tumorigenesis, cell survival, and migration. Gene amplification and overexpression of ACK1 were found in many cancer types such as those of the lung and prostate. Recently, four somatic missense mutations of ACK1, which occur in the N-terminal region, the C-lobe of the kinase domain, and the SH3 domain, were identified in cancer tissue samples.

Synonym(s) : TNK2 , ACK , ACK1 , FLJ44758 , FLJ45547 , p21cdc42Hs

Species(s) : Human

Protein Expression Host : Baculovirus-Insect Cells

Protein Construction : A DNA sequence encoding the amino acid (Gly 110-Trp 476) of human ACK1 isoform 1 (NP_005772.3) was expressed with the GST tag at the N-terminus.

Predicted N-terminal amino acid : Met

Molecular weight/mass : The recombinant human ACK1/GST chimera consists of 592 amino acids and predicts a molecular mass of 68 kDa. It migrates as an approximately 62 kDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the amino acid (Gly 110-Trp 476) of human ACK1 isoform 1 (NP_005772.3) was expressed with the GST tag at the N-terminus.

Formulation : Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerolcerol, 0.5mM EDTA, 0.5mM PMSF, 0.5mM TCEP

Purity : > 90 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet