Human ACE2 (Angiotensin I Converting Enzyme 2) ELISA Kit - E-EL-H0281

Human ACE2 (Angiotensin I Converting Enzyme 2) ELISA Kit

The Human ACE2 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ACE2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ACE2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ACE2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ACE2. You can calculate the concentration of ACE2 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Angiotensin I Converting Enzyme 2

Abbreviation : ACE2

Uniprot ID : Q9BYF1

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.234 ng/ml

Detection Range : 0.391 - 25 ng/ml

Manual & Datasheet

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