Human ABHD4 Protein (His tag) – PKSH031257

Recombinant Human ABHD4 Protein (His tag)

Abhydrolase domain containing 4 (ABHD4), also known as alpha/beta-hydrolase 4 (ABH4), or lyso-N-acylphosphatidylethanolamine lipase, which belongs to the ABHD4/ABHD5 subfamily of peptidase S33 family. Abhydrolase domain containing (ABHD) gene was a small group belongs to alpha/beta hydrolase superfamily. Known members of this group are all found to be involved in important biochemical processes and related to various diseases. The alpha/beta-hydrolase 4 (ABH4) is a lysophospholipase/phospholipase B that selectively hydrolyzes N-acyl phosphatidylethanolamines (NAPEs) and lysoNAPEs. ABH4 accepts lysoNAPEs bearing both saturated and polyunsaturated N-acyl chains as substrates and displays a distribution that closely mirrors lysoNAPE-lipase activity in mouse tissues. The existence of an NAPE-PLD-independent route for NAE biosynthesis and suggest that ABH4 plays a role in this metabolic pathway by acting as a (lyso)NAPE-selective lipase.

Synonym(s) : ABHD4 , ABH4 , FLJ12816

Species(s) : Human

Protein Expression Host : Baculovirus-Insect Cells

Protein Construction : A DNA sequence encoding the full length of human ABHD4 (NP_071343.2) (Met 1-Asp 342) was expressed, with a polyhistidine tag at the N-terminus.

Predicted N-terminal amino acid : His

Molecular weight/mass : The recombinant human ABHD4 consists of 360 amino acids and predicts a molecular mass of 41 kDa. It migrates as an approximately 40 kDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the full length of human ABHD4 (NP_071343.2) (Met 1-Asp 342) was expressed, with a polyhistidine tag at the N-terminus.

Formulation : Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0

Purity : > 82 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet