Human ABCG1 (ATP Binding Cassette Transporter G1) ELISA Kit – E-EL-H0528

Human ABCG1 (ATP Binding Cassette Transporter G1) ELISA Kit

The Human ABCG1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ABCG1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ABCG1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ABCG1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ABCG1. You can calculate the concentration of ABCG1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: ATP Binding Cassette Transporter G1

Abbreviation : ABCG1

Uniprot ID : P45844

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

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