Select Page

Human A2M / CPAMD5 / Alpha-2-macroglobulin Protein (His tag) – PKSH031338

$692.00$1,495.00 CAD

0 in stock (expect 7-21 days for delivery)

Cashback Reward : Earn up to 74$!


SKU: PKSH031338 Category: Tag: Brand:

Recombinant Human A2M / CPAMD5 / Alpha-2-macroglobulin Protein (His tag)

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is an abundant protein of the plasma of vertebrates and members of several invertebrate phyla and functions as a broad-spectrum protease-binding protein. alpha-2-macroglobulin is produced by the liver, and is a major component of the alpha-2 band in protein electrophoresis. alpha-2-macroglobulin is a large plasma glycoprotein that has long been known as an irreversible inhibitor of a variety of proteinases. More recently, it has been reported that numerous growth factors, cytokines and hormones bind to alpha 2M through diverse mechanisms. A2M is also produced in the brain where it binds multiple extracellular ligands and is internalized by neurons and astrocytes. In the brain of Alzheimer’s disease (AD) patients, A2M has been localized to diffuse amyloid plaques. A2M also binds soluble beta-amyloid, of which it mediates degradation. Protease-conjugated alpha2-macroglobulin is selectively bound by cells contacting the body fluids and alpha2-macroglobulin and its protease cargo are then internalized and degraded in secondary lysosomes of those cells. In addition to this function as an agent for protease clearance, alpha2-macroglobulin binds a variety of other ligands, including several peptide growth factors and modulates the activity of a lectin-dependent cytolytic pathway in arthropods.


Synonym(s) : A2mp


Species(s) : Human


Protein Expression Host : Baculovirus-Insect Cells


Protein Construction : A DNA sequence encoding the human A2M (NP_000005.2) (Met 1-Ala 1474) was expressed, fused with a polyhistidine tag at the C-terminus.


Predicted N-terminal amino acid : Ser 24


Molecular weight/mass : The recombinant human A2M consists of 1461 amino acids and predicts a molecular mass of 164 kDa. The apparent molecular mass of rhA2M is approximately 160-170 kDa in SDS-PAGE under reducing conditions.


Protein Construction : A DNA sequence encoding the human A2M (NP_000005.2) (Met 1-Ala 1474) was expressed, fused with a polyhistidine tag at the C-terminus.


Formulation : Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4


Purity : > 92 % as determined by SDS-PAGE


Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method


Stability : Samples are stable for up to twelve months from date of receipt at -70°C


Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.


Reconstitution : Please refer to the printed manual for detailed information.


Manual & Datasheet


Additional information

Format

20 ug, 50 ug

Species

Human

Ask a question about Human A2M / CPAMD5 / Alpha-2-macroglobulin Protein (His tag) – PKSH031338

Reviews for Human A2M / CPAMD5 / Alpha-2-macroglobulin Protein (His tag) – PKSH031338

Reviews

Be the first to review “Human A2M / CPAMD5 / Alpha-2-macroglobulin Protein (His tag) – PKSH031338”