Human 14-3-3 sigma / Stratifin / YWHAS Protein (GST tag) – PKSH031401

Recombinant Human 14-3-3 sigma / Stratifin / YWHAS Protein (GST tag)

14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.

Synonym(s) : YWHAS , SFN , HME1

Species(s) : Human

Protein Expression Host : E. coli

Protein Construction : A DNA sequence encoding the human SFN (NP_006133.1) (Met 1-Ser 248) was fused with the GST tag at the N-terminus.

Predicted N-terminal amino acid : Met

Molecular weight/mass : The recombinant human SFN/GST chimera consists of 480 amino acids and has a predicted molecular mass of 50.1 kDa. It migrates as an approxiamtely 48 kDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the human SFN (NP_006133.1) (Met 1-Ser 248) was fused with the GST tag at the N-terminus.

Formulation : Lyophilized from sterile 20mM Tris, 150mM NaCl, 10mM GSH, 25% glycerol, pH 8.0

Purity : > 94 % as determined by SDS-PAGE

Endotoxin level :

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet