Human 12-LO (Arachidonate 12-Lipoxygenase) ELISA Kit - E-EL-H2445

Human 12-LO (Arachidonate 12-Lipoxygenase) ELISA Kit

The Human 12-LO ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to 12-LO. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for 12-LO and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain 12-LO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of 12-LO. You can calculate the concentration of 12-LO in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Arachidonate 12-Lipoxygenase

Abbreviation : 12-LO

Uniprot ID : P18054

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 - 20 ng/ml

Manual & Datasheet

More information about this Human 12-LO (Arachidonate 12-Lipoxygenase) ELISA Kit from Elabscience