Human β-EP (Beta-Endorphin) ELISA Kit – E-EL-H0572

Human β-EP (Beta-Endorphin) ELISA Kit

The Human β-EP ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with β-EP. During the reaction, Human β-EP in the sample or standard competes with a fixed amount of β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human β-EP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human β-EP in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Beta-Endorphin

Abbreviation : β-EP

Uniprot ID :

Format : 96 rx

Reactivity : Human

ELISA configuration : Competitive

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

More information about this Human β-EP (Beta-Endorphin) ELISA Kit from Elabscience