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His-Tag Monoclonal Antibody – E-AB-20026

  • Clonality : Mouse Monoclonal
  • Applications : WB
  • Reactivity :

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His-Tag Monoclonal Antibody


Background information : Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.


Research area(s) :


Alternate names:


Previous catalog number : ENM0023


Conjugation : none


Clonality : Mouse polyclonal


Clone number : 2K3


Isotype : IgG


Reactivity :


Validated applications : WB


Suggested dilutions : WB 1:500-1:2000


Immunogen : Synthetic Peptide


Swissprot ID :


Gene ID / Accession : /


Calculated MW :


Observed MW :


Purification method : Antigen affinity purification


Supplied in : PBS with 0.02% sodium azide, 50% glycerol pH7.4


Concentration : 1 mg/ml


Storage : Store at -20°C, Avoid freeze / thaw cycles


Other informations :

Link to Elabscience product page


Manual & Datasheet


Additional information

Format

20 ul, 60 ul, 120 ul, 200 ul

Validated Applications

WB

Host

Mouse

Product Name

His-Tag Monoclonal Antibody

Supplier

Clonality

Monoclonal

Conjugation

None

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