Heat shock protein 27, HSP27 Antibody – MCA-6H11

Encor Mouse Monoclonal to Heat shock protein 27 

The heat shock proteins were discovered, as the name suggests, since they are heavily upregulated when cells are stressed by temperatures above the normal physiological range. They are expressed in unstressed cells also and have a normal function as chaperones, helping other proteins to fold correctly, and are required in much greater amounts if the cell or tissue is stressed by heat. The increased levels are generated transcriptionally under the influence of a powerful transcription factor, the heat shock factor 1 (HSF1).
The different heat shock proteins were originally named based on their SDS-PAGE mobility, so HSP27 has an apparent molecular weight of 27 kDa. It is an abundant protein even under non-stress conditions and frequently shows up as a major spot on 2 dimensional gels of cells or tissues. It is known to associate with a variety of other proteins such as actin, intermediate filament subunits and ubiquitin and is found both in the cytoplasm and the nucleus of cells. HSP27 can become heavily phosphorylated under the influence of multiple protein kinases particularly as a result of activation of the p38/SAPK pathway. Upregulation of this protein is protective against neurodegenerative diseases at least in certain mouse models (1). Point mutations in the HSP27 gene are associated with two neurological diseases, Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuropathy IIB (2). These diseases are associated with axonal loss apparently following defects in the transport of neurofilaments. The HGNC name for this protein is HSBP1.

HGNC name(s) : HSBP1

Host : Mouse

Clonality : Monoclonal

ID : EnCor Biotechnology Heat shock protein 27, HSP27 6H11

Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

Isotype : IgG1

Conjugation : none

Immunogen : Recombinant full length human

Mass of detected protein : 27 kDa

Uniprot ID : O75506

KGNC name : HSBP1

RRID # : AB_2572329

Purification : Affinity purified at 1 mg/mL

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions: 

WB: 1:10 000. ICC/IF and IHC: 1:1 000.

Datasheet

Link to on EnCor’s website 

References :

1. Wyttenbach, A et al. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin. Hum. Molec. Genet. 11:1137-1151 (2002).

2. Evgrafov, OV et al. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Nature Genet. 36:602-606 (2004).