GSH (Glutathione) ELISA Kit
The GSH ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with GSH. During the reaction, GSH in the sample or standard competes with a fixed amount of GSH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GSH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.<
Detected Target name: GSH
Abbreviation : GSH
Format : 96 rx
Reactivity : Universal (All Species expexted)
ELISA configuration : Competitive
Sensitivity : 0,938
Detection Range : 1.563-100 μg/ml
More information about this GSH (Glutathione) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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Reactivity | Universal |
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