Green Fluorescent Protein, GFP Antibody – RPCA-GFP

Encor Rabbit Polyclonal Antibody to GFP

Green Fluorescent Protein (GFP) is a ~27 kDa protein isolated originally from the jellyfish Aequoria victoria. It is a fluorescent protein with an excitation maximum at 395 nm and emission maximum at 509 nm, so that when excited with blue or UV light, it emits green light (1). The fluorescence of GFP requires only the polypeptide chain and molecular oxygen and no other cofactors, so it can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell. GFP has been engineered to produce a vast number of variously colored mutants including blue, cyan and yellow protein derivatives, such as BFP, CFP and YFP etc (2-4). GFP and these derivatives are widely used as fluorescent tracers in transfection and transgenic experiments to monitor gene expression and protein localization in Vivo. GFP was the basis of the 2008 Nobel Prize in Chemistry, awarded to Osamu Shimomura, Martin Chalfie and Roger Tsien, specifically “for the discovery and development of the green fluorescent protein, GFP”.

HGNC name(s) : n/a

Host : Rabbit

Clonality : Polyclonal

ID : EnCor Biotechnology Green Fluorescent Protein, GFP GFP

Reactivity : n/a

Isotype : IgG

Conjugation : none

Immunogen : Recombinant full length protein

Mass of detected protein : ~27 kDa

Uniprot ID :

KGNC name : n/a

RRID # : AB_2572317

Purification : Serum, Affinity purified at 1 mg/mL

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1:1 000-5 000
IF/IHC: 1:1 000-5 000

Datasheet

Link to on EnCor’s website

References :

1. Shimomura O, Johnson FH, Saiga Y. Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. Journal of Cellular and Comparative Physiology 3:223–39 (1962).

2. Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ. Crystal structure of the Aequorea victoria green fluorescent protein. Science 273:1392-95 (1996).

3. Heim R, Prasher DC, Tsien RY. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc. Natl. Acad. Sci. USA 91:12501-04 (1994).

4. Lelimousin M, Noirclerc-Savoye M, Lazareno-Saez C, Paetzold B, Le Vot S, Chazal R et al. Intrinsic dynamics in ECFP and Cerulean control fluorescence quantum yield. Biochemistry 48:10038–10046.