GnRH (Gonadotropin-Releasing Hormone) ELISA Kit - E-EL-0071

GnRH (Gonadotropin-Releasing Hormone) ELISA Kit

The GnRH ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with GnRH. During the reaction, GnRH in the sample or standard competes with a fixed amount of GnRH on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to GnRH. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GnRH in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: GnRH

Abbreviation : GNRH1, GRH, HH12, LHRH, LNRH, Luliberin

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 9,375

Detection Range : 15.625-1000 pg/mL

More information about this GnRH (Gonadotropin-Releasing Hormone) ELISA Kit from Elabscience