FlyCut™ Sph I
FlyCut™ Sph I is expressed and purified from E.coli that carries the recombinant SphI gene. The molecular weight is 46.5 kDa, with the recognition site is GCATG^C. The reaction condition is at 37°C, with heat inactivation at 65°C for 20 minutes. This enzyme is not sensitive to dam, dcm methylation or mammalian CpG methylation.
Sph I Enzyme Properties
Fast cleavage in 5-10 minutes with high fidelity
FlyCut™ SphI components:
- FlyCut™ SphI : 250 u / 500 u
- 10x FlyCut™ Buffer: 1 ml / 1 ml
- 10x DNA Loading Buffer: 500 ul / 1 ml
Manual & Datasheet:
Additional information
Format | 250 u, 500 u |
---|---|
Supplier |
Reviews