FlyCut™ Nhe I – JN301

FlyCut NheI

FlyCut™ Nhe I is expressed and purified from E.coli that carries the recombinant NheI gene. The molecular weight is 39.1 kDa, with the recognition site at G^CTAGC. The reaction condition is at 37 °C, with heat inactivation at 80 °C for 20 minutes. This restriction enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.

Nhe I Restriction Enzyme Properties

Fast cleavage in 5-10 minutes with high fidelity

FlyCut NheI components:

• FlyCut™ Nhe I : 500 u / 1 000 u
• 10x FlyCut™ Buffer: 1 ml / 1 ml
• 10x DNA Loading Buffer: 1 ml / 1 ml

Manual & Datasheet

JN301 FlyCutTM NheI