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FlyCut™ Nhe I – JN301

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FlyCut NheI

FlyCut™ Nhe I is expressed and purified from E.coli that carries the recombinant NheI gene. The molecular weight is 39.1 kDa, with the recognition site at G^CTAGC. The reaction condition is at 37 °C, with heat inactivation at 80 °C for 20 minutes. This restriction enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.


Nhe I Restriction Enzyme Properties

Fast cleavage in 5-10 minutes with high fidelity


FlyCut NheI components:

  • FlyCut™ Nhe I : 500 u / 1 000 u
  • 10x FlyCut™ Buffer: 1 ml / 1 ml
  • 10x DNA Loading Buffer: 1 ml / 1 ml

Manual & Datasheet

JN301 FlyCutTM NheI

Additional information

Format

1 000 u, 500 u

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