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Flag-Tag Polyclonal Antibody – E-AB-20003

  • Clonality : Rabbit Polyclonal
  • Applications : WB | ELISA
  • Reactivity :

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Flag-Tag Polyclonal Antibody


Background information : Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. 


Research area(s) :


Alternate names: DDDDK epitope tag, DDDDK epitope tag, DYKDDDDK epitope tag


Previous catalog number : ENG004


Conjugation : none


Clonality : Rabbit polyclonal


Clone number :


Isotype : IgG


Reactivity :


Validated applications : WB | ELISA


Suggested dilutions : WB 1:500-1:2000, ELISA 1:5000-1:20000


Immunogen : DDDDK synthetic peptide conjugated to KLH.


Swissprot ID :


Gene ID / Accession : /


Calculated MW :


Observed MW : 35 kDa


Purification method : Antigen affinity purification


Supplied in : PBS with 0.02% sodium azide, 50% glycerol and 0.5% BSA


Concentration : 1 mg/ml


Storage : Store at -20°C. Avoid freeze / thaw cycles.


Other informations :

Link to Elabscience product page


Manual & Datasheet


Additional information

Format

20 ul, 60 ul, 120 ul, 200 ul

Validated Applications

WB, ELISA

Host

Rabbit

Product Name

Flag-Tag Polyclonal Antibody

Supplier

Clonality

Polyclonal

Conjugation

None

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