Flag-Tag Polyclonal Antibody – E-AB-20003
- Clonality : Rabbit Polyclonal
- Applications : WB | ELISA
- Reactivity :
169.00$ – 599.00$ CAD
Flag-Tag Polyclonal Antibody
Background information : Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies.
Research area(s) :
Alternate names: DDDDK epitope tag, DDDDK epitope tag, DYKDDDDK epitope tag
Previous catalog number : ENG004
Conjugation : none
Clonality : Rabbit polyclonal
Clone number :
Isotype : IgG
Validated applications : WB | ELISA
Suggested dilutions : WB 1:500-1:2000, ELISA 1:5000-1:20000
Immunogen : DDDDK synthetic peptide conjugated to KLH.
Swissprot ID :
Gene ID / Accession : /
Calculated MW :
Observed MW : 35 kDa
Purification method : Antigen affinity purification
Supplied in : PBS with 0.02% sodium azide, 50% glycerol and 0.5% BSA
Concentration : 1 mg/ml
Storage : Store at -20°C. Avoid freeze / thaw cycles.
Other informations :
20 ul, 60 ul, 120 ul, 200 ul
Flag-Tag Polyclonal Antibody
Ask a question about Flag-Tag Polyclonal Antibody – E-AB-20003
Product Reviews for Flag-Tag Polyclonal Antibody – E-AB-20003
More to Read
Which is the Best Kit for DNA Clean Up? Our aim was to perform a PCR Purification Kit Comparison from different vendors. At Civic Bioscience, we distribute the Favorprep™ and EasyPure® product lines from FAVORGEN and TransBionovo respectively. First, the FavorPrep™...
The Problem with Antibodies used for Research Antibodies used for Research in academic, industrial and therapeutic fields are universal reagents in molecular biology assays. Without them, the entire field of medical research could collapse. However, there are many...
GC-rich PCR: FastPfu FLY vs Q5® High-Fidelity DNA Polymerase GC-rich PCR can be difficult to achieve Our aim was to verify compare FastPfu FLY (TransBionovo) and Q5 (NEB) high-fidelity DNA Polymerase at amplifying a GC-rich sequence (human ARX; 79% GC) from purified...
What's the Detection Limit of Taq ? How many DNA copies from gDNA? There are MANY Taq DNA Polymerases out there on the market. Some are driven by strong marketing and others by strong performance. Our aim was to verify the Detection Limit of Taq DNA Polymerases in PCR...
by Simon Roy | Sep 16th, 2019 | Latest News, Promotions |This offer is not available yet. Please check again tomorrow. 20% discount on selected cell biology products: TransDetect® Cell Counting Kit (CCK)-FC101 TransDetect® Annexin V-FITC/PI Cell Apoptosis...
A huge THANK YOU for your support! MERCI encore INFINIMENT de supporter notre jeune compagnie! by Simon Roy | September 16th, 2019 | Company Posts, Latest NewsPrices for services vary case by case. 4rth anniversary promotions are coming soon! Let's CELEBRATE...
DNA Polymerases | EasyTaq | FastPfu | PCR | PCR Purification | Taq DNA PolymeraseSave on the Products that MatterOur already popular 222$ DUO Miniprep + Gel/PCR Purification now allows you to choose 2 x 300rx minipreps or 2x 300rx Gel/PCR purification. Already...
For my own birthday, every year, I find a way to celebrate with you. To be quite frank, the promotion I want to do is, for the moment, not configurable on the website but I will work on it in the coming days so that it becomes possible by Valentine’s Day. However, I...