EasyTaq® DNA Polymerase for PAGE – AP112

EasyTaq® DNA Polymerase for PAGE

EasyTaq® DNA Polymerase for PAGE is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase for PAGE has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. lt lacks 3′-5′ exonuclease activity. This enzyme is supplied with an unique buffer formulation without BSA, therefore its PCR product is suitable for use in SDS-PAGE and agarose gel electrophoresis.

Advantages EasyTaq DNA Polymerase for PAGE

• The extension rate is about 1-2 kb/min.
• Specially formulated without BSA.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 3 kb.
• Available with or without 2.5 mM dNTPs.

Product Manual and Datasheet

EasyTaq® DNA Polymerase for PAGE