Easy II Protein Quantitative Kit (BCA) – DQ111
$96.00 CAD
0 in stock (expect 7-21 days for delivery)
Easy II Protein Quantitative Kit (BCA)
The BCA protein assay is one of the most commonly used methods for protein quantification. Under alkaline condition, the reduction of Cu2+ to Cu+ is realized by peptide bonds in proteins (biuret reaction). The amount of reduced copper is directly proportional to the amount of total proteins. Bicinchoninic acid (BCA) is a chromogenic reagent that chelates the reduced copper, producing a stable purple complex. Compared to Bradford assay, BCA protein assay is more objective, chromogenic reaction can occur on general peptide bonds. It is compatible with high concentration of SDS (5%), Triton X-100 (5%), NP 40 (5%), CHAPS (5%), Tween 20, 60, 80 (5%). Some reagents, including reducing agents (e.g. dithiothreitol and β-mercaptoethanol), chelating agents (EDTA, EGTA) and high-concentration buffers, interfere with the reactions. However, these interferences can be eliminated by diluting the protein samples.
Contains BSA standard solution (0.2 mg/l) 2 x 1 ml.
Manual and datasheet
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Format | 1 kit |
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