E2 (Estradiol) ELISA Kit – E-EL-0065

E2 (Estradiol) ELISA Kit

The E2 ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with Goat Anti-Rabbit IgG, make solid-phase secondary antibody. And then add sample,horseradish peroxidase-labeled estradiol and anti-estradiol antibody, so as toform a coated secondary antibody – anti-estradiol antibody – HRP-labeled Estradiol complex. The amount of bound labeled estradiol is inversely proportional to the amount of estradiol in the sample. TMB substrate solutionis added to each well after wash the microtiter plate, TMB turn to blue catalystwith horseradish peroxidase (HRP). The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to thestandard curve.<

Detected Target name: E2

Abbreviation : 17β-Estradiol, 17beta-Estradiol, Oestradio, Beta-Estradiol

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 13,125

Detection Range : 21.875-1400 pg/mL

More information about this E2 (Estradiol) ELISA Kit from Elabscience