DJ1 Antibody – MCA-4H4

Encor Mouse Monoclonal to DJ1

DJ-1, also known as Park7, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ1 superfamily that function as protein chaperones, catalases, proteases and kinases. DJ-1 was originally cloned as an oncogene that cooperatively transforms cells together with H-ras (1). Mutations in the DJ-1 gene are associated with rare forms of autosomal recessive early-onset Parkinson’s disease (2). DJ-1 may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death (3-5). Augmenting DJ-1 activity might provide novel approaches to treating chronic neurodegenerative illnesses such as Parkinson’s disease and acute damage such as stroke (6).
MCA-4H4 was generated against full length recombinant human DJ-1 expressed in and purified from E. coli. This antibody binds to the human and bovine DJ1 proteins, but does not recognize the rat and mouse DJ-1 proteins. The bovine protein is about 96% identical to the human protein, while the rodent proteins are about 91% similar. The HGNC name for this protein is PARK7.

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HGNC name(s) : PARK7

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Host : Mouse

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Clonality : Monoclonal

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ID : EnCor Biotechnology DJ1 4H4

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Reactivity : Human | Horse | Cow | Pig | Rat | Mouse

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Isotype : IgG1

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Conjugation : none

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Immunogen : Recombinant full length human

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Mass of detected protein : 21 kDa

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Uniprot ID : Q99497

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KGNC name : PARK7

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RRID # : AB_2572260

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Purification : Affinity purified at 1 mg/mL

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Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

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Validated applications : WB | IF/ICC | IHC

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Suggested Dilutions:

WB: 1:5 000.
IF/IHC: 1:5 000 .

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Datasheet

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Link to on EnCor’s website

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References :

1. Nagakubo D, Taira T, Kitaura H, Ikeda M, Tamai K, Iguchi-Ariga SM and Ariga H DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras. Biochem. Biophys. Res. Commun. 231, 509–513 (1997).

2. Bonifati V, Rizzu P, van Baren, M.J., Schaap O, Breedveld GJ, Krieger E, Dekker MC, Squitieri F, Ibanez P, Joosse M et al. Mutations in the DJ-1 gene associated with autosomal recessive early-onset Parkinsonism. Science, 299, 256–259 (2003).

3. Xu J, Zhong N, Wang H, Elias JE, Kim CY, Woldman I, Pifl C, Gygi SP, Geula C, Yankner BA. The Parkinson’s disease-associated DJ-1 protein is a transcriptional co-activator that protects against neuronal apoptosis. Hum Mol Genet. 14 (9):1231-41 (2005)

4. Yokota T, Sugawara K, Ito K, Takahashi R, Ariga H. And Mizusawa, H. Down regulation of DJ-1 enhances cell death by oxidative stress, ER stress, and proteasome inhibition. Biochem. Biophys. Res. Commun., 312, 1342–1348 (2003).

5. Taira T, Saito Y, Niki T, Iguchi-Ariga SM, Takahashi K and Ariga H. DJ-1 has a role in antioxidative stress to prevent cell death. EMBO Rep., 5, 213–218 (2004).

6. Bonifati V, Oostra BA. and Heutink, P. Linking DJ-1 to neurodegeneration offers novel insights for understanding the pathogenesis of Parkinson’s disease. J.Mol.Med.82,163-174 (2004).

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