DHVD3 (1,25-Dihydroxyvitamin D3) ELISA Kit – E-EL-0016

DHVD3 (1,25-Dihydroxyvitamin D3) ELISA Kit

The DHVD3 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to DVD/DHVD3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for DVD/DHVD3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DVD/DHVD3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of DVD/DHVD3. You can calculate the concentration of DVD/DHVD3 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: DHVD3

Abbreviation : Calcitriol,1,25-dihydroxycholecalciferol , 1,25-dihydroxyvitamin D3,1,25-(OH)2D3 , 1,25(OH)2D

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 4,688

Detection Range : 7.813-500 pg/mL

More information about this DHVD3 (1,25-Dihydroxyvitamin D3) ELISA Kit from Elabscience