Chicken FGF23 (Fibroblast Growth Factor 23) ELISA Kit – E-EL-Ch2200

Chicken FGF23 (Fibroblast Growth Factor 23) ELISA Kit

The Chicken FGF23 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FGF23. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FGF23, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of FGF23. You can calculate the concentration of FGF23 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Chicken Fibroblast Growth Factor 23

Abbreviation : FGF23

Uniprot ID : R4GIR0

Format : 96 rx

Reactivity : Chicken

ELISA configuration : Sandwich

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

More information about this Chicken FGF23 (Fibroblast Growth Factor 23) ELISA Kit from Elabscience